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iCell Bioscience Inc
cell culture medium [dmem: 70%; m3:basef (cat. no. m300f-500; incell llc)]: 20%; fbs: 10 Cell Culture Medium [Dmem: 70%; M3:Basef (Cat. No. M300f 500; Incell Llc)]: 20%; Fbs: 10, supplied by iCell Bioscience Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/cell culture medium [dmem: 70%; m3:basef (cat. no. m300f-500; incell llc)]: 20%; fbs: 10/product/iCell Bioscience Inc Average 90 stars, based on 1 article reviews
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Cellgro
phenol red-free medium (dmem/ham's f-12, cat# 10-092cv) Phenol Red Free Medium (Dmem/Ham's F 12, Cat# 10 092cv), supplied by Cellgro, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/phenol red-free medium (dmem/ham's f-12, cat# 10-092cv)/product/Cellgro Average 90 stars, based on 1 article reviews
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Corning Life Sciences
dmem (dulbecco’s modified eagle’s medium) /hams f-12 50/50 mix media (cat # 16-405-cv) Dmem (Dulbecco’s Modified Eagle’s Medium) /Hams F 12 50/50 Mix Media (Cat # 16 405 Cv), supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/dmem (dulbecco’s modified eagle’s medium) /hams f-12 50/50 mix media (cat # 16-405-cv)/product/Corning Life Sciences Average 90 stars, based on 1 article reviews
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GeneDireX Inc
l-arginine free dmem medium cat # cc927-0500 ![]() L Arginine Free Dmem Medium Cat # Cc927 0500, supplied by GeneDireX Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/l-arginine free dmem medium cat # cc927-0500/product/GeneDireX Inc Average 90 stars, based on 1 article reviews
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Keygen Biotech
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Corning Life Sciences
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VivaCell Biotechnology GmbH
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HiMedia Laboratories
dulbecco’s modified eagle’s medium (dmem) (cat no. al001a) ![]() Dulbecco’s Modified Eagle’s Medium (Dmem) (Cat No. Al001a), supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/dulbecco’s modified eagle’s medium (dmem) (cat no. al001a)/product/HiMedia Laboratories Average 90 stars, based on 1 article reviews
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Image Search Results
Journal: Frontiers in Immunology
Article Title: The regulatory role of eosinophils in adipose tissue depends on autophagy
doi: 10.3389/fimmu.2023.1331151
Figure Lengend Snippet: ATG5 deficiency in eosinophils induces Arg2 expression, resulting in decreased beiging in adipocytes. (A, B) RNA-seq. The volcano plot of differentially expressed genes (DEGs) in bone marrow (A) and blood (B) -isolated eosinophils from Atg5 eoΔ Il5 tg was compared to bone marrow and blood-isolated eosinophils from Ctrl Il5 tg . A total of 13 and 25 DEGs were identified, respectively. (C) Quantitative PCR. The isolation of eosinophils from bone marrow (BM) and blood of Ctrl II5 tg and Atg5 eoΔ II5 tg mice was followed by gene expression analysis for Arg2 and Atg5 . The expression of the target genes was normalized using Actb and Nono as reference genes (n=3). (D) Immunoblotting. Protein lysates from freshly isolated BM and blood-derived eosinophils of Ctrl II5 tg and Atg5 eoΔ II5 tg mice were subjected to analysis for ATG5 and ARG2 protein expression. ACTB protein levels were utilized as loading controls. (E) Arginase activity assay. Eosinophils freshly isolated from BM and blood samples of Ctrl II5 tg and Atg5 eoΔ II5 tg mice were subjected to an arginase activity assay (n = 3). (F) Immunoblotting. Protein lysates were obtained from mature adipocytes incubated in L-arginine free DMEM supplemented with different L-arginine concentrations for five hours. ACTB protein levels served as loading controls. (G) Immunoblotting. Eosinophils were cultured in the presence and absence of ABH-hydrochloride, and their supernatants were applied to adipocytes, followed by UCP1 immunoblotting. (H) Quantitative PCR. qPCR was performed on adipocytes that underwent the same treatments described in the previous section to evaluate the expression level of thermogenic genes. Expression levels were normalized using Actb and Nono as reference genes and compared to control mice. (n = 3). Values are means ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001.
Article Snippet: To assess the effect of different concentrations of L-arginine, mature adipocytes incubated in
Techniques: Expressing, RNA Sequencing, Isolation, Real-time Polymerase Chain Reaction, Gene Expression, Western Blot, Derivative Assay, Arginase Activity Assay, Incubation, Cell Culture, Control